Biohydrogen production from wastes of plant and animal origin via dark fermentation
Abstract
This study investigated the batch experiments on biohydrogen production from wastes of plant and animal origin. Several substrates including sugar beet pulp (SBP), sugar beet leaves (SBL), sugar beet stillage (SBS), rye stillage (RS), maize silage (MS), fruit and vegetable waste (FVW), kitchen waste (KW) and slaughterhouse waste (SHW) including intestinal wastes, meat tissue, post flotation sludge were tested for their suitability for hydrogen production. Generally, the substrates of plant origin were found to be appropriate for dark fermentation, and the highest hydrogen yield of 280 dm3 H2/kg VS was obtained from fruit and vegetable waste. Contrary to these findings, slaughterhouse waste as well as kitchen waste turned out to be unsuitable for hydrogen production although their methane potential was high. It was also concluded that the combined thermal pretreatment with substrate acidification was needed to achieve high hydrogen yields from wastes.
Keyword : biohydrogen, plant biomass, food waste, dark fermentation, anaerobic digestion
How to Cite
Cieciura-Włoch, W., & Borowski, S. (2019). Biohydrogen production from wastes of plant and animal origin via dark fermentation. Journal of Environmental Engineering and Landscape Management, 27(2), 101-113. https://doi.org/10.3846/jeelm.2019.9806
This work is licensed under a Creative Commons Attribution 4.0 International License.
References
Akobi, C., Hafez, H., & Nakhla, G. (2016). The impact of furfural concentrations and substrate-to-biomass ratios on biological hydrogen production from synthetic lignocellulosic hydrolysate using mesophilic anaerobic digester sludge. Bioresource Technology, 221, 598-606. https://doi.org/10.1016/j.biortech.2016.09.067
Angelidaki, I., & Ahring, B. K. (1992). Effects of free long-chain fatty-acids on thermophilic anaerobic digestion. Applying Microbiology and Biotechnology, 37, 808-812. https://doi.org/10.1007/BF00174850
Angelidaki, I., Alves, M., Bolzonella, D., Borzacconi, L., Campos, J. L., Guwy, A. J., Kalyuzhnyi, S., Jenicek, P., & van Lier, J. B. (2009). Defining the biomethane potential (BMP) of solid organic wastes and energy crops: a proposed protocol for batch assays. Water Science & Technology, 59(5), 927-934.
Argun, H., & Dao, S. (2017). Bio-hydrogen production from waste peach pulp by dark fermentation: Effect of inoculums addition. International Journal of Hydrogen Energy, 26, 2569-2574. https://doi.org/10.1016/j.ijhydene.2016.06.225
Benito Martin, P. C., Schlienz, M., & Greger, M. (2017). Production of bio-hydrogen and methane during semi-continuous digestion of maize silage in a two-stage system. International Journal of Hydrogen Energy, 42, 5768-5779. https://doi.org/10.1016/j.ijhydene.2017.01.020
Chojnacka, A., Błaszczyk, M. K., Szczęsny, P., Nowak, K., Sumińska, M., Tomczyk-Żak, K., Zielenkiewicz, U., & Sikora, A. (2011). Comparative analysis of hydrogen-producing bacterial biofilms and granular sludge formed in continuous cultures of fermentative bacteria. Bioresource Technology, 102, 10057-10064. https://doi.org/10.1016/j.biortech.2011.08.063
Chu, C.-F., Li, Y.-Y., Xu, K.-Q., Ebie, Y., Inamori, Y., Kong, H.-N. (2008). A pH- and temperature-phased two-stage process for hydrogen and methane production from food waste. International Journal of Hydrogen Energy, 33, 4739-4746. https://doi.org/10.1016/j.ijhydene.2008.06.060
Cuetos, M. J., Gómez, X., Otero, M., & Morán, A. (2010). Anaerobic digestion and co-digestion of slaughterhouse waste (SHW): Influence of heat and pressure pre-treatment in biogas yield. Waste Management, 30, 1780-1789. https://doi.org/10.1016/j.wasman.2010.01.034
Dredge, R., van Dyk, S. J., Radloff, S. E., & Pletschke, B. I. (2011). Lime pretreatment of sugar beet pulp and evaluation of synergy between ArfA, ManA and XynA from Clostridium cellulovorans on the pretreated substrate. Biotechnology, 3, 151-159. https://doi.org/10.1007/s13205-011-0019-3
Escamilla-Alvarado, C., Rios-Leal, E., Ponce-Noyola, M. T., & Poggi-Varaldo, H. M. (2012). Gas biofuels from solid substrate hydrogenogenic-methanogenic fermentation of the organic fraction of municipal solid waste. Process Biochemistry, 47, 1572-15878. https://doi.org/10.1016/j.procbio.2011.12.006
Fang, H. H. P., Zhang, T., & Liu, H. (2002). Microbial diversity of a mesophilic hydrogen producing sludge. Applying Microbiology and Biotechnology, 5, 112-118. https://doi.org/10.1007/s00253-001-0865-8
Ghimire, A., Frunzo, L., Pirozzi, F., Trably, E., Escudie, R., Lens, P. N. L., & Esposito, G. (2015). A review on dark fermentative biohydrogen production from organic biomass: Process parameters and use of by-products. Applying Energy, 144, 73-95. https://doi.org/10.1016/j.apenergy.2015.01.045
Guo, X. M., Trably, E., Latrinne, E., Carrere, H., & Steyer, J. P. (2010). Hydrogen production from agricultural waste by dark fermentation: A review. International Journal of Hydrogen Energy, 35, 10660-10673. https://doi.org/10.1016/j.ijhydene.2010.03.008
Hallenbeck, P. C. (2009). Fermentative hydrogen production: Principles, progress, and prognosis. International Journal of Hydrogen Energy, 34, 7379-7389. https://doi.org/10.1016/j.ijhydene.2008.12.080
Lay, J., Fan, K., Hwang, J., Cgng, J., & Hsu, P. (2005). Factors affecting hydrogen production from food wastes by Clostridium-rich composts. Journal Environmental Energy, 131(4), 595-602.
Lee, D.-Y., Ebie, Y., Xu, K.-Q., Li, Y.-Y., & Inamori, Y. (2010). Continuous H2 and CH4 production from high-solid food waste in the two-stage thermophilic fermentation process with the recirculation of digester sludge. Bioresource Technology, 101, 42-47. https://doi.org/10.1016/j.biortech.2009.03.037
Li, M., Zhao, Y., Guo, Q., Qijan, X., & Niu, D. (2008). Bio-hydrogen production from food waste and sewage sludge in the presence of aged refuse excavated from refuse landfill. Renewable Energy, 33 (12), 2573-2579. https://doi.org/10.1016/j.renene.2008.02.018
Li, Y., & Jin, Y. (2015). Effects of thermal pretreatment on acidification phase during two-phase batch anaerobic digestion of kitchen waste. Renewable Energy, 77, 550-557. https://doi.org/10.1016/j.renene.2014.12.056
Luo, G., Xie, L., Zou, Z. H., Zhou, Q., & Wang, J. Y. (2010). Fermentative hydrogen production from cassava stillage by mixed anaerobic microflora: Effects of temperature and pH. Applying Energy, 87, 3710-3717. https://doi.org/10.1016/j.apenergy.2010.07.004
Noike, T., Takabatake, H., Mizuno, O., & Ohba, M. (2002). Inhibition of hydrogen fermentation of organic wastes by lactic acid bacteria. International Journal of Hydrogen Energy, 27, 1367-1371. https://doi.org/10.1016/S0360-3199(02)00120-9
O-Thong, S., Prasertsana, P., Intrasungkhab, N., Dhamwichukornc, S., & Birkelandd, N. K. (2008). Optimization of simultaneous thermophilic fermentative hydrogen production and COD reduction from palm oil mill effluent by Thermoanaerobacterium-rich sludge. International Journal of Hydrogen Energy, 33, 1221-1231. https://doi.org/10.1016/j.ijhydene.2007.12.017
Ozkan, L., Erguder, T. H., & Demirer, G. N. (2011). Effect of pretreatment methods on solubilization of beet-pulp and biohydrogen production yield. International Journal of Hydrogen Energy, 36, 382-389. https://doi.org/10.1016/j.ijhydene.2010.10.006
Panagiotopoulos, J. A., Bakker, R. R., de Vrije, T., Urbaniec, K., Koukiose, G., & Claassen, P. A. M. (2010). Prospects of utilization of sugar beet carbohydrates for biological hydrogen production in the EU. Journal Cleaner Production, 18, 9-14. https://doi.org/10.1016/j.jclepro.2010.02.025
Pattra, S., Sangyoka, S., Boonmee, M., & Reungsang, A. (2008). Bio-hydrogen production from the fermentation of sugarcane bagasse hydrolysate by Clostridium butyricum. International Journal of Hydrogen Energy, 33, 5256-5265. https://doi.org/10.1016/j.ijhydene.2008.05.008
Pawar, S. S., Nkemka, V. N., Zeidan, A. A., Murto, M., & van Niel, E. W. J. (2013). Biohydrogen production from wheat straw hydrolysate using Caldicellulosiruptor saccharolyticus followed by biogas production in a two-step uncoupled process. International Journal of Hydrogen Energy, 38, 9121-9130. https://doi.org/10.1016/j.ijhydene.2013.05.075
Ren, N., Xing, D., Rittmann, B. E., Zhao, L., Xie, T., & Zhao, X. (2007). Microbial community structure of ethanol type fermentation in bio-hydrogen production. Environmental Microbiology, 9, 1112-1125. https://doi.org/10.1111/j.1462-2920.2006.01234.x
Rice, E. W., Baird, R. B., Eaton, A. D., & Clesceri, L. S. (Eds.). (2012). Standard methods for the examination of water and wastewater (22nd ed.). American Public Health Association, Washington, DC.
Saady, N. M. C. (2013). Homoacetogenesis during hydrogen production by mixed cultures dark fermentation: Unresolved challenge. International Journal of Hydrogen Energy, 38, 13172-13191. https://doi.org/10.1016/j.ijhydene.2013.07.122
Santos, S. C., Rosa, P. R. F., Sakamoto, I. K., Varesche, M. B. A., & Silva, E. L. (2014). Continuous thermophilic hydrogen production and microbial community analysis from anaerobic digestion of diluted sugar cane stillage. International Journal of Hydrogen Energy, 39, 9000-9011. https://doi.org/10.1016/j.ijhydene.2014.03.241
Sikora, A., Błaszczyk, M., Jurkowski, M., & Zielenkiewicz, U. (2013). Lactic acid bacteria in hydrogen-producing consortia: On purpose or by coincidence? In M. Kongo (Ed.), Lactic acid bacteria − R & D for food, health and livestock purposes (pp. 487-514). Rijeka, Croatia: Intech. https://doi.org/10.5772/50364
Tsapekos, P., Kougiasp, G., Treu, L., Campanaro, S., & Angelidaki, I. (2017). Process performance and comparative metagenomic analysis during co-digestion of manure and lignocellulosic biomass for biogas production. Applying Energy, 185, 126-135. https://doi.org/10.1016/j.apenergy.2016.10.081
Urbaniec, K., & Bakker, R. R. (2015). Biomass residues as raw material for dark hydrogen fermentation – A review. International Journal of Hydrogen Energy, 40, 3648-3658. https://doi.org/10.1016/j.ijhydene.2015.01.073
Urbaniec, K., & Grabarczyk, R. (2014). Hydrogen production from sugar beet molasses – a techno-economic study. Journal Cleaner Production, 65, 324-329. https://doi.org/10.1016/j.jclepro.2013.08.027
Wicher, E., Seifert, K., Zagrodnik, R., Pietrzyk, B., & Łaniecki, M. (2013). Hydrogen gas production from distillery wastewater by dark fermentation. International Journal of Hydrogen Energy, 38, 7767-7773. https://doi.org/10.1016/j.ijhydene.2013.04.008
Xia, A., Cheng, J., & Murphy, J. D. (2016). Innovation in biological production and upgrading of methane and hydrogen for use as gaseous transport biofuel. Biotechnology Advances, 34, 451-472. https://doi.org/10.1016/j.biotechadv.2015.12.009
Yang, P., Zhang, R., McGarvey, J. A., & Benemann, J. R. (2007). Biohydrogen production from cheese processing wastewater by anaerobic fermentation using mixed microbial communities. International Journal of Hydrogen Energy, 32, 4761-4771. https://doi.org/10.1016/j.ijhydene.2007.07.038
Zhong, W., Zhang, Z., Luo, Y., Sun, S., Qiao, W., & Xiao, M. (2011). Effect of biological pretreatments in enhancing corn straw biogas production. Bioresource Technology, 102, 1117711182. https://doi.org/10.1016/j.biortech.2011.09.077
Angelidaki, I., & Ahring, B. K. (1992). Effects of free long-chain fatty-acids on thermophilic anaerobic digestion. Applying Microbiology and Biotechnology, 37, 808-812. https://doi.org/10.1007/BF00174850
Angelidaki, I., Alves, M., Bolzonella, D., Borzacconi, L., Campos, J. L., Guwy, A. J., Kalyuzhnyi, S., Jenicek, P., & van Lier, J. B. (2009). Defining the biomethane potential (BMP) of solid organic wastes and energy crops: a proposed protocol for batch assays. Water Science & Technology, 59(5), 927-934.
Argun, H., & Dao, S. (2017). Bio-hydrogen production from waste peach pulp by dark fermentation: Effect of inoculums addition. International Journal of Hydrogen Energy, 26, 2569-2574. https://doi.org/10.1016/j.ijhydene.2016.06.225
Benito Martin, P. C., Schlienz, M., & Greger, M. (2017). Production of bio-hydrogen and methane during semi-continuous digestion of maize silage in a two-stage system. International Journal of Hydrogen Energy, 42, 5768-5779. https://doi.org/10.1016/j.ijhydene.2017.01.020
Chojnacka, A., Błaszczyk, M. K., Szczęsny, P., Nowak, K., Sumińska, M., Tomczyk-Żak, K., Zielenkiewicz, U., & Sikora, A. (2011). Comparative analysis of hydrogen-producing bacterial biofilms and granular sludge formed in continuous cultures of fermentative bacteria. Bioresource Technology, 102, 10057-10064. https://doi.org/10.1016/j.biortech.2011.08.063
Chu, C.-F., Li, Y.-Y., Xu, K.-Q., Ebie, Y., Inamori, Y., Kong, H.-N. (2008). A pH- and temperature-phased two-stage process for hydrogen and methane production from food waste. International Journal of Hydrogen Energy, 33, 4739-4746. https://doi.org/10.1016/j.ijhydene.2008.06.060
Cuetos, M. J., Gómez, X., Otero, M., & Morán, A. (2010). Anaerobic digestion and co-digestion of slaughterhouse waste (SHW): Influence of heat and pressure pre-treatment in biogas yield. Waste Management, 30, 1780-1789. https://doi.org/10.1016/j.wasman.2010.01.034
Dredge, R., van Dyk, S. J., Radloff, S. E., & Pletschke, B. I. (2011). Lime pretreatment of sugar beet pulp and evaluation of synergy between ArfA, ManA and XynA from Clostridium cellulovorans on the pretreated substrate. Biotechnology, 3, 151-159. https://doi.org/10.1007/s13205-011-0019-3
Escamilla-Alvarado, C., Rios-Leal, E., Ponce-Noyola, M. T., & Poggi-Varaldo, H. M. (2012). Gas biofuels from solid substrate hydrogenogenic-methanogenic fermentation of the organic fraction of municipal solid waste. Process Biochemistry, 47, 1572-15878. https://doi.org/10.1016/j.procbio.2011.12.006
Fang, H. H. P., Zhang, T., & Liu, H. (2002). Microbial diversity of a mesophilic hydrogen producing sludge. Applying Microbiology and Biotechnology, 5, 112-118. https://doi.org/10.1007/s00253-001-0865-8
Ghimire, A., Frunzo, L., Pirozzi, F., Trably, E., Escudie, R., Lens, P. N. L., & Esposito, G. (2015). A review on dark fermentative biohydrogen production from organic biomass: Process parameters and use of by-products. Applying Energy, 144, 73-95. https://doi.org/10.1016/j.apenergy.2015.01.045
Guo, X. M., Trably, E., Latrinne, E., Carrere, H., & Steyer, J. P. (2010). Hydrogen production from agricultural waste by dark fermentation: A review. International Journal of Hydrogen Energy, 35, 10660-10673. https://doi.org/10.1016/j.ijhydene.2010.03.008
Hallenbeck, P. C. (2009). Fermentative hydrogen production: Principles, progress, and prognosis. International Journal of Hydrogen Energy, 34, 7379-7389. https://doi.org/10.1016/j.ijhydene.2008.12.080
Lay, J., Fan, K., Hwang, J., Cgng, J., & Hsu, P. (2005). Factors affecting hydrogen production from food wastes by Clostridium-rich composts. Journal Environmental Energy, 131(4), 595-602.
Lee, D.-Y., Ebie, Y., Xu, K.-Q., Li, Y.-Y., & Inamori, Y. (2010). Continuous H2 and CH4 production from high-solid food waste in the two-stage thermophilic fermentation process with the recirculation of digester sludge. Bioresource Technology, 101, 42-47. https://doi.org/10.1016/j.biortech.2009.03.037
Li, M., Zhao, Y., Guo, Q., Qijan, X., & Niu, D. (2008). Bio-hydrogen production from food waste and sewage sludge in the presence of aged refuse excavated from refuse landfill. Renewable Energy, 33 (12), 2573-2579. https://doi.org/10.1016/j.renene.2008.02.018
Li, Y., & Jin, Y. (2015). Effects of thermal pretreatment on acidification phase during two-phase batch anaerobic digestion of kitchen waste. Renewable Energy, 77, 550-557. https://doi.org/10.1016/j.renene.2014.12.056
Luo, G., Xie, L., Zou, Z. H., Zhou, Q., & Wang, J. Y. (2010). Fermentative hydrogen production from cassava stillage by mixed anaerobic microflora: Effects of temperature and pH. Applying Energy, 87, 3710-3717. https://doi.org/10.1016/j.apenergy.2010.07.004
Noike, T., Takabatake, H., Mizuno, O., & Ohba, M. (2002). Inhibition of hydrogen fermentation of organic wastes by lactic acid bacteria. International Journal of Hydrogen Energy, 27, 1367-1371. https://doi.org/10.1016/S0360-3199(02)00120-9
O-Thong, S., Prasertsana, P., Intrasungkhab, N., Dhamwichukornc, S., & Birkelandd, N. K. (2008). Optimization of simultaneous thermophilic fermentative hydrogen production and COD reduction from palm oil mill effluent by Thermoanaerobacterium-rich sludge. International Journal of Hydrogen Energy, 33, 1221-1231. https://doi.org/10.1016/j.ijhydene.2007.12.017
Ozkan, L., Erguder, T. H., & Demirer, G. N. (2011). Effect of pretreatment methods on solubilization of beet-pulp and biohydrogen production yield. International Journal of Hydrogen Energy, 36, 382-389. https://doi.org/10.1016/j.ijhydene.2010.10.006
Panagiotopoulos, J. A., Bakker, R. R., de Vrije, T., Urbaniec, K., Koukiose, G., & Claassen, P. A. M. (2010). Prospects of utilization of sugar beet carbohydrates for biological hydrogen production in the EU. Journal Cleaner Production, 18, 9-14. https://doi.org/10.1016/j.jclepro.2010.02.025
Pattra, S., Sangyoka, S., Boonmee, M., & Reungsang, A. (2008). Bio-hydrogen production from the fermentation of sugarcane bagasse hydrolysate by Clostridium butyricum. International Journal of Hydrogen Energy, 33, 5256-5265. https://doi.org/10.1016/j.ijhydene.2008.05.008
Pawar, S. S., Nkemka, V. N., Zeidan, A. A., Murto, M., & van Niel, E. W. J. (2013). Biohydrogen production from wheat straw hydrolysate using Caldicellulosiruptor saccharolyticus followed by biogas production in a two-step uncoupled process. International Journal of Hydrogen Energy, 38, 9121-9130. https://doi.org/10.1016/j.ijhydene.2013.05.075
Ren, N., Xing, D., Rittmann, B. E., Zhao, L., Xie, T., & Zhao, X. (2007). Microbial community structure of ethanol type fermentation in bio-hydrogen production. Environmental Microbiology, 9, 1112-1125. https://doi.org/10.1111/j.1462-2920.2006.01234.x
Rice, E. W., Baird, R. B., Eaton, A. D., & Clesceri, L. S. (Eds.). (2012). Standard methods for the examination of water and wastewater (22nd ed.). American Public Health Association, Washington, DC.
Saady, N. M. C. (2013). Homoacetogenesis during hydrogen production by mixed cultures dark fermentation: Unresolved challenge. International Journal of Hydrogen Energy, 38, 13172-13191. https://doi.org/10.1016/j.ijhydene.2013.07.122
Santos, S. C., Rosa, P. R. F., Sakamoto, I. K., Varesche, M. B. A., & Silva, E. L. (2014). Continuous thermophilic hydrogen production and microbial community analysis from anaerobic digestion of diluted sugar cane stillage. International Journal of Hydrogen Energy, 39, 9000-9011. https://doi.org/10.1016/j.ijhydene.2014.03.241
Sikora, A., Błaszczyk, M., Jurkowski, M., & Zielenkiewicz, U. (2013). Lactic acid bacteria in hydrogen-producing consortia: On purpose or by coincidence? In M. Kongo (Ed.), Lactic acid bacteria − R & D for food, health and livestock purposes (pp. 487-514). Rijeka, Croatia: Intech. https://doi.org/10.5772/50364
Tsapekos, P., Kougiasp, G., Treu, L., Campanaro, S., & Angelidaki, I. (2017). Process performance and comparative metagenomic analysis during co-digestion of manure and lignocellulosic biomass for biogas production. Applying Energy, 185, 126-135. https://doi.org/10.1016/j.apenergy.2016.10.081
Urbaniec, K., & Bakker, R. R. (2015). Biomass residues as raw material for dark hydrogen fermentation – A review. International Journal of Hydrogen Energy, 40, 3648-3658. https://doi.org/10.1016/j.ijhydene.2015.01.073
Urbaniec, K., & Grabarczyk, R. (2014). Hydrogen production from sugar beet molasses – a techno-economic study. Journal Cleaner Production, 65, 324-329. https://doi.org/10.1016/j.jclepro.2013.08.027
Wicher, E., Seifert, K., Zagrodnik, R., Pietrzyk, B., & Łaniecki, M. (2013). Hydrogen gas production from distillery wastewater by dark fermentation. International Journal of Hydrogen Energy, 38, 7767-7773. https://doi.org/10.1016/j.ijhydene.2013.04.008
Xia, A., Cheng, J., & Murphy, J. D. (2016). Innovation in biological production and upgrading of methane and hydrogen for use as gaseous transport biofuel. Biotechnology Advances, 34, 451-472. https://doi.org/10.1016/j.biotechadv.2015.12.009
Yang, P., Zhang, R., McGarvey, J. A., & Benemann, J. R. (2007). Biohydrogen production from cheese processing wastewater by anaerobic fermentation using mixed microbial communities. International Journal of Hydrogen Energy, 32, 4761-4771. https://doi.org/10.1016/j.ijhydene.2007.07.038
Zhong, W., Zhang, Z., Luo, Y., Sun, S., Qiao, W., & Xiao, M. (2011). Effect of biological pretreatments in enhancing corn straw biogas production. Bioresource Technology, 102, 1117711182. https://doi.org/10.1016/j.biortech.2011.09.077